Fig. 8. Changes in Akt and AMPKα1/2 phosphorylation (A and C) and Nf-kB and PPARγ expression (B and D) in Huh7.5 cells. Densitometric analysis and an example of Western blot taken from 5 different experiments are shown. AMPKα1/2: AMP-activated protein kinase α1/2; Nf-kB: nuclear factor kappa-light-chain-enhancer of activated B cells; PPARγ: peroxisome proliferator activating ligand receptors γ; G: genistein; E: 17β-estradiol. C=control (untreated cells); FFAs=free fatty acids (2 mM for 3 h). The data were normalized versus control value. Reported data are means ± SD of three independent experiments for each experimental protocol. Significance among groups: * P <0.05 vs. C; # P <0.05 vs FFAs.